How should I dispose of silver staining waste (SDS-PAGE)?

I know I cannot pour silver staining waste down the sink, but how should I dispose of it? Thank you in advance!

18 November 2020 1,745 3 View

Do you know a not very expensive journal of Metagenomics?

Hi you all, I'm looking for a not very expensive journal to submit our work related to microbial diversity in a beverage drink using an amplicon perspective. Any suggestion is welcome because I...

02 May 2020 2,165 4 View

Can you help me with a lab protocol (RT-PCR, capillary electrophoresis, etc) to detect the fusion gene FIP1L1-PDGFRa in hypereosinophilia disorders?

I need a molecular biology protocol to detect this fusion gene FIP1L1-PDGFRa in hypereosinophilia disorders, because the protocol that I have is not working, and I see several inespecifical bands...

13 February 2020 1,304 4 View

Did anyone of you ever resuspended compounds such as beta sitosterol or campesterol in culture medium?

I noticed that these compunds are not soluble in DMSO, and DMSO damages hepatocarcinoma cells a lot. Thank you!

04 February 2020 6,575 1 View

How many mice do I need to do qPCR of T cells sorted from B16 TILs?

03 February 2020 9,215 2 View

Why is the TMB substrate turning blue without being in contact with the sample and in the dark?

Hi everyone, last time I was doing an ELISA without exposing it to the light and before I added the substrate it was turning blue. Do you have any idea or suggestion of a better substrate?, the...

03 January 2020 1,294 7 View

How to prevent 68Ga-colloid formation?

Hi, I am currently trying to optimize the radiolabeling of a peptide (w/ NOTA) but I am ending up with 30% 68Ga-colloid in the reaction. I am using HEPES as the buffer and setting the reaction pH...

06 October 2019 1,181 2 View

How can i know if my cell type expresses a certain type of enzyme?

In an informatic database, like typing the name of the enzyme and then the information of the enzymes expressed in that particular cell type.

16 May 2019 2,893 3 View

What antibody is used for the E. histolytica amebiasis antibody test(blood)?

I am trying to see if I can test the serum of infected mice with this antibody

15 January 2019 8,257 6 View

Is my panel data set appropriate for GMM?

I have a panel data set with N=22, T=15 Although N>T, is my N=22 appropriate for GMM estimations? Thanks

10 November 2018 806 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

How to generate AA6061 alloy or any aluminium alloy crystal in material studio?

I need to carry out molecular dynamics simulation on Aluminium alloy. For this i need the crystal structure that which could be further replicated to form a structure.

22 February 2021 1,410 1 View

How to examine multicolineairty in backwards regression with binary variables?

I did a backwards regression with various binary variables, but some of the binary variables are negatively correlated with the outcome, which would make no sense cause the correlation univariate...

21 February 2021 4,392 3 View

Plant research: Watering uniformity/consistency. How important is it?

Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...

21 February 2021 6,389 3 View