Will i have to prepare different standard curve for each drug conc. loaded in nanoparticles or one standard curve is enough for different drug conc. loaded?

1. You are taking 1 ml from 10 ml and again make up the volume by fresh 1 ml then there is no change in dilution factor and still the dilution factor is 10. Dilution factor depends on the volume in which your nanoparticles is suspended. Removal and addition of fixed volume of solvent didn't alter the dilution factor, it is only to maintain the sink condition.

2. At first you have to make a standard curve and find out the amount of drug loaded in it. Suppose you are taking 10 mg of drug at the time of loading, but not all the drugs are loaded on nanocarrier, so you have to find out the loaded amount by the help of standard curve.

3. After each release take absorbance spectra and calculate the release amount by standard curve then multiply it with dilution factor.

4. Amount of drug released = conc * dilution factor

5. % drug release= amount of released drug/ loaded drug amount x 100

for more details follow my research paper

Article Stability and acidic pH-mediated leakage of guest molecules ...

Ravinder Kumar

You are right, there is need to have a reference spectrum for each concentration.

Thank you Avinash Kumar. I think it will help.