Hi All,

I was wondering if anyone knew of any correlation analyses comparing the size of CA1 pyramidal cells and frequency of spontaneous activity. Doing the eye test, it seems like I’m getting increased rates of sIPSCs onto CA1 pyramidal as the cell size increases (measured by capaticance).

Additionally, I was curious about the best way to interpret miniature (with TTx) IPSC frequency data. If the frequency is increased compared to control, can you conclude that there are increased numbers of synaptic connections or that the vesicular release probability is increased or both? My gut tells me it is both and I’d have to do other experiments to tease apart the exact mechanism...ie paired-pulse for release probability and some sort of synaptic labeling and counting for increased synaptic number.

Thanks,

Chad