Please I urgently need assistance on what concentration (Unit/ml) of the enzyme (using HHL as substrate) can be used in a spectrophotometric detection (quantification) of the product (hippuric acid) formed.
I only have 0.1UN of the ACE (obtained from Sigma) for the over all assay.
However, some authors have used as low as 0.5 and 3mUnit/ml of the enzyme using HPLC method of quantification.
But I am Using spectrophotometric Method.
Thanks for your kind assistance