Please I urgently need assistance on what concentration (Unit/ml) of the enzyme (using HHL as substrate) can be used in a spectrophotometric detection (quantification) of the product (hippuric acid) formed.

I only have 0.1UN of the ACE (obtained from Sigma) for the over all assay.

However, some authors have used as low as 0.5 and 3mUnit/ml of the enzyme using HPLC method of quantification.

But I am Using spectrophotometric Method.

Thanks for your kind assistance

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