Spectinomycin concentration

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Zebrafish Cell Culture?

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How do you determine at what concentration of antimicrobial should you subject the pathogen to?

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Is it normal to use Tris buffer when testing for bacteriostatic properties of isolated compounds?

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Pankaj Agnihotri

Dear you could make 100mg/ml stock and could use this stock as 100ug/ml final concentration

Sigit Ismawanto

Thank you for your information.

By the way

Do you have any experience in gene construct preparation by using Gateway technology??

Tien Yin Yau

Gateway isn't evil, Invitrogen is evil. Gateway is a great technology though as long as you don't mind paying for the clonase. I've found that it works really efficently and consistantly is is particularly useful if I need to put a single construct into several different targets (i.e. lentiviral vector and transient mammalian expression vector). I agree with Aaron with the additional point that if you're PCRing with attB-flanked primers, make sure you're using a proofreading pol.

David Majerowicz

I'm experience with Gateway technology is the worst possible... I couldn't make even the first recombination...