Hello,
I'm trying to find suitable antibodies to discriminate hepatocytes from other cell types in the mouse fetal liver. I was considering ASGR1, but I found that it can be expressed in monocytes as well, or ABCC2/MRP2, but I couldn't find a nice antibody (please help in case you know one).
Any good (fetal) hepatocytes marker would be good for me.
In an ideal world, I would prefer a conjugated antibody against a surface marker for flow cytometry of live cells, but also an unconjugated antibody for immunofluorescence (in this case it can be an intracellular marker). I want to confidently distinguish hepatocytes from other cell types such as endothelial and haematopoietic cells.
Many thanks!
Hello Giovanni Canu
You can use CD13 and DLK1 as cell surface markers for hepatocytes in mouse fetal liver. Please refer to the research article attached under the heading, Identification of novel hepatic markers.
Article Comparative analysis of cell lineage differentiation during ...
Best.
See the following useful article:
Article Purification and chracterization of mouse fetal liver epithe...
I would use a multiparameter approach as many markers are expressed by multiple cell types. Use TER119 and CD45 to identify and eliminate hematopoietic cells. Don't overlook autofluorescence and light scatter to help identify large cells, versus the many smaller cell types.
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