Specific antibody to recognize cerulean?

More Sophie Brameyer's questions See All

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Does anyone have experience with AML/T cell cultures in serum-free medium?

We want to keep AML cell lines together with T cells in serum free medium for about 4-7 days. Currently we culture them in RPMI 1640 with 10% FCS, so something resembling that would be good I...

22 May 2024 1,763 0 View

Employees willing to participate in interview of max 15 minutes?

I am a master student at Erasmus university and writing my thesis about branding in the banking sector. I am desperately looking for participants for my interviews, a lot of companies are too busy...

02 April 2024 3,264 1 View

How do you quantify cells in coronal mouse sections using ImageJ?

Hi everyone, We are trying to count the number of cells within a ROI in the Dentate Gyrus from fluorescently labeled mouse brain tissue (sectioned at 40 microns). We have samples with cells that...

20 December 2023 7,869 0 View

How does the split-packaging system with lentiviruses work during virus production in eukaryotic cells?

I still don't figure it out yet how exactly a virus particle is formed by adding three plasmids (transfer plasmid with transgene, packaging plasmid and envelope plasmid) in a cell by...

27 November 2023 5,725 4 View

Bubbles/Sparkles in Sections for Device Placement Confirmation?

My lab is experiencing a new issue with several mouse brain tissue sections (for confirming placement, no immunohistochemistry). Each tissue section has white "sparkles" that dissipate over time...

12 November 2023 1,757 0 View

Does a validated Medical Student Self-Efficacy Scale exist in French?

The primary objective of this inquiry is to establish the existence of a validated Medical Student Self-Efficacy Scale in the French language. This question is motivated by the significance of...

31 October 2023 5,676 0 View

Can anyone provide help with inconsistent results MAIPS4510 ELISPOT plates (Merck, Millipore)?

I am having trouble getting consistente results with MAIPS4510 ELISPOT plates (Merck, Millipore). We have used them before with no trouble and using the same protocol. Or positive controls (PHA)...

26 October 2023 7,248 0 View

Are there alternatives for a CO2 incubator with O2 regulation ?

Dear all, I'm looking for an alternative for a CO2 incubator with O2 regulation as these are quite expensive and I actually just need 5% O2, 5% CO2, 37°C over 3 days every 2 weeks (should fit...

10 April 2023 5,395 2 View

How can I decide on mixed methods sample size (parallel design) for a pilot study?

Hi, I'm working on a research proposal for a pilot study and struggling to land on the optimal sample size. The quant measures (physiological measures and surveys) are recorded while the...

27 March 2023 9,408 6 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

15 July 2024 6,008 5 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

What are the criteria that must be retained for the development and validation of a qualitative NMR method ?

Dear Community, I would like to develop and validate a qualitative NMR method for the analysis of a specific category of chemicals, and my question is the following: What are the criteria that I...

08 July 2024 1,402 4 View

Do anyone experiment occasional staining problems with cryoprotectant containing Polyvinyl-pyrrolidone and Ethylene glycole?

Occasionally we observe lack of staining in the inner part of a skin section processed with indirect immunofluorescence. Can be due to incomplete removal of the cryoprotectan during the wash? Any...

07 July 2024 8,200 2 View

Can I use BSA for a Histological Blocking Solution?

Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...

07 July 2024 7,967 3 View