@ Deviprasad, the specific protein present in the nitrogen fixing rhizobium species are a catalytic iron-dependent protein, commonly referred to as MoFe protein and a reducing iron-only protein (Fe protein) . A regulatory protein encoded by rosR was identified to characterize the rhizobium. Identification of various Rhizobium species can also be achieved through a conventional nodulation assay, which requires growing a host plant inoculated with the Rhizobium species.