Sorting early apoptotic thymocytes ?

19 February 2018 1 3K Report

For an experiment I am thinking of sorting early apoptotic thymocytes. Does anyone have any experience with this ? I am a bit worried that the sorting process will turn my cells late apoptotic/necrotic. If anyone has any advice/tips/protocols they would be most welcome.

Cheers,

pieter

Emmanuel Ifeanyi Obeagu

Dear Louwe, Chloromethyl-x-rosamine is an aldehyde-fixable potential-sensitive fluorochrome for the detection of early apoptosis. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. It can be done by combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS).

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