Dear friends,
We are now following the 15 mL glass tubes for Blood collection and clotting for 1 hour at RT. This is yielding very less serum quantity and seems like we are loosing bit quantity as hemolysis too. I would like to have the best protocol that yields a better serum separation and less hemolysis. Also please specify the material requirements also so that the the yields can be improved.
Depending on the volume you need, you may wish to use smaller tubes to get a better central clot and to allow it to retract from the wall of the tube. Putting it in the refrigerator too soon slows clotting. Are your collecting anti-serum or serum for other assays? Be careful with syringes so that you do not put too much pressure on the syringe when emptying it into the tube. If using Vacutainers, keep the bottom of the tube lower than the needle.
Thanks everyone. I have tried the procedure of Clotting and storing in 4 degrees overnight . this procedure yields better non hemolyzed serum. Thanks for the share of knowledge and your valuable time. Appreciated. :)