I usually work with primary hepatocites from rats. Sometime I use HEPG2 or Huh7 for my experiments, but these are cell lines from hepatomas, so I'd like a human hepatocyte cell line from non tumoral cells.
ATCC Human Liver Hepatocytes Immortalized by SV40 Large T-Antigen
https://www.atcc.org/Products/All/PTA-5565.aspx?slp=1
Please see:
http://www.upcyte.com/products/human-upcyte-hepatocytes.html
and the following publications:
Alexandra Burkard , Caroline Dähn , Stefan Heinz , Anne Zutavern , Vera Sonntag-Buck , Daniel Maltman , Stefan Przyborski , Nicola J. Hewitt , Joris Braspenning: Generation of proliferating human hepatocytes using upcyte® technology: characterisation and applications in induction and cytotoxicity assays. Xenobiotica Vol. 42, Iss. 10, 2012
Natalie Herzog, Max Hansen, Sebastian Miethbauer, Kai-Uwe Schmidtke, Ursula Anderer, Amelie Lupp, Sebastian Sperling, Daniel Seehofer, Georg Damm, Katrin Scheibner, Jan-Heiner Küpper: Primary-like Human Hepatocytes Genetically Engineered to Obtain Proliferation Competence Display Hepatic Differentiation Characteristics in Monolayer and Organotypical Spheroid Cultures. Cell Biology International 12/2015; DOI:10.1002/cbin.10574
Mi dispiace, ma non ho mai lavorato su questa tipologia di cellule.
Actually, the suggested choice don't suit me completely.
Thank you all, anyway ;-)
Hello,
ABM has a collection of hepatocytes (https://www.abmgood.com/productSearch.php?searchQuery=hepatocytes)
You can give them a try.
There is a Primary Human Hepatocytes Webinar coming up that may be helpful and answer any additional questions.
Title: Current State and Future Prospects for Primary Human Hepatocytes in Basic Research Applications Presenter: Edward L. LeCluyse, Ph.D. Principal Scientist, LifeNet Health
Date: June 5, 2018 Duration: 60 minutes Times: 1PM (EST) / 12 PM (CST) To register for the event, visit: SigmaAldrich.com/PHHWebinar
What you will learn:
- Current challenges with the use of primary hepatocytes for basic research applications
- The state of the art in tissue procurement and hepatocyte isolation techniques; Improved measures for the characterization and validation of primary hepatocytes lots for specific research applications
- Efforts towards creating universal standards and quality metrics for the use of human tissues and cells for basic research applications
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- Biological Science
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