Are there any public datasets of consumer complaints about service industries at the European level?

06 January 2021 1,718 2 View

Is it allowed to apply Kubelka Munk function (1-R)^2/2R to Vis diffuse reflectance spectra on a glass powder?

Reflectance spectra are performed on the plane surface of a compact glass powder disk (5 mm thickness). The glass particles can range from 20 to 100 micrometers. The instrument is a portable...

21 July 2020 4,577 2 View

I need to do a top file or a prm file for Chlorogenic acid with an OPLSAA force field. Thus, how can I create these files?

I am trying to simulate the interaction between this acid and a protein, so I am following the steps of the protein-ligand Gromacs tutorial. However, I need a prm file to complete the complex...

27 November 2019 9,460 1 View

Does anyone have experience installing PEBL on Linux?

Dear colleagues I'm planning to run an experiment using the Psychology Experiment Building Language (PEBL) but when I try to install the software using "wine" to run it on Linux, I get the...

09 October 2018 928 1 View

Is XRD the best technique to differentiate graphitic carbon from organic carbon and carbonates?

Hello. I am interested on graphite characterization. Is XRD the best technique to differentiate graphitic carbon from organic carbon and carbonates? Which other techniques can you suggest me? Can...

31 July 2018 6,416 4 View

Why PDA medium change its colour to orange?

Hello, I isolate different fungi from soil, one of them can change colour of PDA to orange. Does anyone know why? Thanks you in advice Merry christmas and happy new year :) M. Carmen

28 December 2017 2,069 4 View

What is the best culture medium to isolate Gliocladium spp. from soil?

03 October 2017 8,784 4 View

Transformation of an angular variable?

Which transformation should I apply to an angular variable (i.e. slope of a landslide) in order to normalize it (like arcsin to percentages)? Even though ANOVA are robust to not normal...

27 February 2017 8,585 2 View

Post hoc test for a contingency table NxN?

When I run a contingency table NxN and I get a significant result with the chi-square test, is there a post-hoc test in SPSS to address the comparisons? Or should I run logistic...

27 February 2017 2,858 1 View

Could you please provide references concerning the regulation of plant life cycle?

Wisconsin fast plants (Brassica rapa) are the product of a breeding program developed for decades. They grow to full size in about 28-30 days. What has changed in them respect to their original...

13 April 2016 3,638 18 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View