I'd like to know whether Zeb1 protein is detectable by means of immunohistochemistry on pDCs or not.
I have identified many solutions. I need suggestion from somebody with application experience of this topic to identify the most reliable and robust procedure.
21 July 2024 3,465 5 View
Hello everyone, I have a question. Assuming I have a 200 kg hydrogen tank at 200 bar, and assuming I can't go below a pressure of 20 bar. How can I calculate the mass of hydrogen that left the system?
06 June 2024 2,216 2 View
Good morning I have a question. How can I calculate the distributed pressure drops for a compressed gases in pipe? For Example, hydrogen compressed at 30 bar with a flow of 195 Nm^3/h. In a UHP...
17 March 2024 5,572 5 View
Please note the my new mail address is "[email protected]"
06 March 2024 6,554 0 View
I have many files ROI regionsXband(84x7). I would like to visualize it on sLoreta but when i convert the file txt into Slor, the output is empty and i can't visualize it. Anyone know how resolve it?
31 January 2024 2,996 0 View
Hello, we have been struggling with the lots of background in our wb membranes probed with an anti-Streptavidin-HRP from Thermofisher (Pierce 21134). Samples contained biotinilated proteins. Every...
16 October 2023 3,778 2 View
Dear Colleagues, I have been measuring the area of tropical fern leaves for several species, however the perimeter is a different and difficult task. Ferns have higlhy divided and the small...
06 September 2023 3,680 2 View
Hello fellow Abaqus users, I've been working with Abaqus for the past two years and have encountered a significant issue in my simulations related to the plastic deformation of sheet metals....
06 September 2023 3,663 4 View
I'm having a really hard time finding software to help me analyze calcium sparks. I use VSM cells and the fluo-4 am calcium indicator, I record 30s (80 frames) in the LSM780 confocal, each...
02 August 2023 4,094 3 View
Hi, I am currently working on a device tailored for the Pharmaceutical industry and CROs. While it is most suited for utilization within a hood with horizontal laminar flow, I have only...
10 May 2023 6,152 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View