I recently read about methods of resuspending exosomes in 5% glucose for in vivo.
have someone try or know what the logic behind it?
maybe it's only related to the electroporation?
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Hi, We accidentally bought Nessler's Reagent A (potassium tetraiodomercurate(II) solution) instead of Nessler's Reagent (for detection of ammonia). I understand that we can add NaOH at a certain...
28 May 2022 7,956 4 View
Hi, Im trying to isolate intratestinal macrophages from mouse lungs, and grow them in culture for a few days, to collect secretome. Does somebody have a good protocol for it? I tried face...
11 May 2022 1,039 3 View
I think, determination is the most influencing trait of an entrepreneur, how we can assess this quality in controlled environment.
06 April 2022 8,224 2 View
I see that Blaiser, K. & Lamb, B. (2012) presented the Acoustic Monitoring Protocol, at the American Speech-Language Hearing Association Convention. Atlanta, Georgia, but is there a...
06 September 2021 5,645 0 View
Dear RG community members, I use Adobe Illustrator for many years - https://www.adobe.com/products/illustrator.html and I are now looking for alternatives. The Adobe Illustrator is great, of...
04 March 2021 8,688 6 View
My research includes monitoring canopy temperature over time with a thermal FLIR camera. I am looking for a code/program that will help me analyze the videos easily and automatically. For my...
24 February 2020 4,893 3 View
I'm plating copper with nickel (electroless). Then, I'm doing porosity test according to the procedure below. I can't find the exact reactions going on between the ferrocyanide and the copper...
27 December 2019 7,084 0 View
Hi, I've been using growthcurver package for growth curves experiment. I have the output data per well- but now I want to group wells together according to my meta-data (for instance three...
21 June 2019 6,772 1 View
Hi, I have a CRISPR system based on two AAV vectors 1/2, the titers are 10^12 for the gRNA and 10^10 for the Cas9. I'm having troubles in the proccess of primary neurons transduction - I can't...
20 May 2019 6,861 6 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?
02 August 2024 9,518 2 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.
28 July 2024 6,501 0 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View