Someone evaluating the impact of COVID-19 on patients with an arbovirus infection?

20 April 2020 0 8K Report

I would like to know about the relationship between COVID-19 and the various arboviruses, mainly dengue, zika and chikungunya.

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Why is linearity somewhat lost when plotting the Stern-Volmer (SV) graph?

When preparing a graph of fluorophore intensity vs. analyte concentration, the linearity shows an R² of 0.999. However, when plotting the same data as Fo/F vs. analyte concentration, the linearity...

13 July 2024 7,131 0 View

Question about ELISA with the "Cattle IGF 1 (Insulin like growth factor) ELISA Kit" Cat: ELK5630?

Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...

13 June 2024 9,473 0 View

What is the best method for quantifying lipid accumulation in C. elegans images using ImageJ?

We have a demarcation and analysis protocol in ImageJ for images of C. elegans stained with Oil Red O. However, it does not seem to be the best way and we were unable to find an easy-to-execute...

15 April 2024 2,031 1 View

Best way to prescribe periodic boundary conditions (PBC) in Abaqus/Explicit?

Hello, I'm looking to conduct simulations of RVEs (unidirectional discontinuous carbon fibers in resin matrix) utilizing the embedded finite elements technique within Abaqus. I've been successful...

09 April 2024 5,575 1 View

What is the typical % of CD54 and CD86 positive cells in THP-1 cells exposed to DNCB?

DNCB is used as a standard reactivity test for THP-1 cells and supposed to lead to an increase in RFI of at least 200% and 150% respectively. However, I do not seem to find the actual % of...

07 April 2024 3,416 0 View

Problems with conventional pcr. What can it be?

I have a problem with conventional PCR electrophoresis, we are analyzing the deletion of ccr5 delta 32. And this material started to cause problems recently, there was no change in the protocol...

03 April 2024 3,896 3 View

Is it possible to use mean-field homogenisation in Abaqus with Dynamics Implicit?

Greetings, I'm interested in whether it's possible to employ mean-field homogenization (MFH) within Abaqus alongside the Dynamics Implicit (or Explicit) solver. I'm exploring this possibility as...

26 March 2024 3,969 4 View

How to perform a statistical bias correction for wind speed with small in situ time series?

Hi! I wish to perform some kind of bias correction for my historical wind speed time series, which comes from ERA5. However, I just have a few measurements of wind at 100 meters coming from the...

20 March 2024 8,503 0 View

Can anyone share a protocol for quantifying lipids in C. elegans with oil red?

I have already carried out the lipid staining test in C. elegans with oil red, however, in the last tests I am unable to stain all the animals efficiently, most of them do not stain or only have...

22 January 2024 8,696 2 View

Why does the solution containing ivermectin crystallize over time?

11 January 2024 2,140 0 View

E-commerce businesses, COVID-19 and Entrepreneurial Strategies (innovation, proactiveness and risk-taking)?

Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey

05 August 2024 5,332 3 View

Why not we are following sars COVID 19 guidance?

If the case of Irak is of December 2020 then i think I can suggest pl follow all the infection cause and death, already in our researchgate.

19 July 2024 3,356 2 View

Use of NC or NTC to descibe negative control?

Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...

18 July 2024 4,183 2 View

Dengue in Southern states in India?

There is rapid surge of dengue cases in Southern parts of India. However there are no cases till now in the setting I work in Northern India. Besides dengue surveillance and local preventive...

14 July 2024 2,935 2 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

How to load Remedesivir drug into mesoporous nanoparticles?

Hello, I want to load Remedesivir (RNA polymerase inhibitor) into mesoporous nanoparticles (MSNs). The issue is drug is temperature and pH sensitive and it degrades quickly. I need advice on its...

01 July 2024 889 3 View

Is distance education effective as Onsite education?

Since the experience of COVID-19 in the world, educational bodies, institute of higher learning has adopted distance education. Even though the use has existed ever before the COVID-19 issue....

22 May 2024 7,430 3 View

Assistant with TUNEL technique for A549 cells, switch from trypsinized cells to adherent cells?

Hi, Im using the kit from abcam (ab66108) In situ Direct DNA Fragmentation (TUNEL) Assay Kit for A549 cells, the kit required to trypsinize the cells which its okay (in theory) but the problem is...

07 May 2024 4,706 0 View

What are Trends in viral infection among cancer patients?

Hello every one Can any one suggest me trends subjects in viral infection in cancer patients for PHD thesis? Thanks in advance.

05 May 2024 5,263 3 View

How to obtain high yield of flavivirus in cells?

Please, give advices how to reach high virus titer in cell culture after infection. Cultural liquid after this will be used to next infections and i need advices or protocols to achieve high...

22 April 2024 8,193 2 View