Some one help, How can I induce glioblastomas in rats?

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How may i find a researcher who is working on Teachers Engagement?

Hi everyone, my current Ph.D Project is on "Teachers Engagement in STEM teaching" Please I like to link up with (find) another or any researcher working on Teachers Engagement. I will be grateful...

14 January 2021 8,170 5 View

What Explained Variance is good to proceed with Predictive Soil Mapping?

I have 1851 soil samples data on pH covering a study area of 7482sq.km in northern Ghana and I am using 52 environmental long-term average variables (Relief, Climate, MODIS Reflectances and...

27 December 2020 2,728 6 View

How did you feel when you received a notification of the acceptance of your first paper?

As a novice in the world of research with the aim to publish in a peer-reviewed high-quality journal, you do all you can to get quality work. Sometimes the feedback from reviewers can discourage...

09 September 2020 147 31 View

Is it possible to get DOI for previous publications without DOIs?

Have previous publications without DOI with certain journals though they now provide DOIs but don't seems to be ready to link up for the provision of DOIs for previous publications. Any means to...

31 August 2020 9,773 5 View

Reference style "Games for Health Journal"?

Does anybody knows if there is a reference style in Mendeley for "Games for Health Journal" ISSN 21617856, 2161783X?

09 July 2020 8,151 1 View

How do i get a cancer cell and standard procedure for storage?

I may need a standard procedure of storage

05 April 2020 163 3 View

OK to store pelleted transformed E coli at 4C for 2 days?

Immediately after transforming competent cells with a plasmid ordered from Addgene, I began growing a mini prep culture (5ml) overnight. Next morning, I pelleted the cells and put them in the...

01 February 2020 2,085 2 View

What is the primary structure of a typical keynote address usually delivered at a conference?

Keynote address

07 September 2019 567 7 View

Is adult learning really receiving attention and support globally?

We are living in the world of fast-paced changes, and thanks to ICT. It is really easing learning, especially among the the young adults. That notwithstanding, adult learning seem not to really...

01 March 2019 522 13 View

For people doing top-down proteomics, what has been a reliable volatile buffer system in your preparation of intact protein analytes?

Attempting to produce spectra of SPDP (527 Da adduct on solvent exposed lysines) modified transferrin. I am experiencing solubility issues even at the recommended concentration of 10uM with a...

30 July 2018 3,791 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

How to do rat bone Immunofluorescence, Please suggest?

Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo

26 January 2021 4,165 3 View

Taijun Hana

Is not it enough using xenograft model? For ex, U87 injected rats?

As for the injection part, brain is best but subcutaneous is also OK.

Safa Azar

to know if the tumor was induced you can label your cell line before injection into the rats with luciferase and then you can follow tumor growth and distribution using bioluminescence technic .

example: https://bio-protocol.org/e1762