Methods for selecting the best CHO clone?

I'm going to be sub cloning CHO cells but I need some advice on what to look for. These clones will then enter an AMBR 250 for selection of best performing clone, where we will pick the best and...

15 March 2020 2,172 2 View

Has anyone done Gibson Assembly to build a large DNA construct?

Dear all, I am currently trying to do a 3-way Gibson Assembly using 3 DNA fragments of size, 2.4 kb, 4 kb and 6.3 kb. The final ligated product will have a size of 12 kb. My protocol involves...

08 January 2018 4,181 5 View

Choice of lacI repressor gene?

Dear all, I am working on a synthetic biology project and will be using a titratable expression system (pLac). Since this system gives leaky expression, I would like to introduce a lacI repressor...

11 January 2016 3,267 9 View

Do I have to include a LacI repressor region when using an inducible T7 promoter?

Dear all, I am currently working on a synthetic biology project where I have to construct my own plasmid for protein expression. In regards with protein expression, I have chosen the inducible T7...

05 February 2015 6,904 3 View

How do I exclude internal promoters?

Dear all, I am now working with a bacterial gene cluster and located 26 internal promoters within this cluster. Most of these promoters are found on coding region and I plan to remove them for...

27 October 2014 430 8 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

Best excel chart to compare algorithms' running time?

Hi I want to compare the running time of robotic path planning algorithms. The grid size is 8X8. I have 100 scenarios of start and goal positions.. Each scenario is run in three obstacles ratio...

22 February 2021 621 1 View

How to open an old ".MTS" file containing spectroscopic data?

Hi supreme hive-mind of the internet, we have some old *.MTS files that are supposed to contain spectroscopic data, probably some coordinates and a long list of single line spectra. Basically,...

16 February 2021 9,385 2 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View

How to check if the PMF profile obtained from ABF simulation is correct?

I am doing ABF simulations to calculate ligand binding enegy. The PMF profile that I get using single long ABF simulation is attached as pmf_total.png. When I do this ABF calculation with 10...

10 February 2021 4,170 2 View

What is the Greedy windowed algorithm description?

Hi Dear Ph. D., I have carefully read your paper on anomaly detection in smart meters. Unfortunately, the research does not decribe the greedy windowed algorithm, neither its complexity, nor the...

10 February 2021 2,462 3 View