As lyophilization involved removal of water (or volatile liquid) from a product, a solution with solely its water content removed should be able to reconstitute back into desirable concentration.
Recently I have synthesized an organic compound (no salt) with pH7.4. The lyophilized compound does NOT soluble in either water nor oils (the temperatures are set to 95 degree C). What could possibly be the reason?
Your comment will be very much appreciated.
Hello sir, this may be attributed to the change in solubility profile of the organic compound on lyophilisation. This indicates the highly sensitive nature of the organic compound to a critical processing step like lyophilisation. Sir, you can try its solubility in organic solvents. Furthermore the changes in the compound structural features can be studied. One of the most obvious reason seems to be the sensitivity of the organic compound to freezing or any or all(in synergism) steps involved in lyophilisation.
Possible reasons must be two:
a. A polymorphism (deb therefore be water soluble, but are different equilibration times)
b. You have another compound: Perform a melting point and HPLC ...
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