If a chemical is soluble in 30 mg in 1 ml100% ethanol or 100% DMSO, how can i dilute it without affecting the solubility and to avoid toxicity on living organisms?
Hum. Solubility is the maximum mass of a solid that you can dissolve in a certain mass ( ou volume) of a solvent in a certain temperature. If your substance dissolves 30 mg in 1 ml ethanol ( or 30 g/liter). So, If you add more solvent It Will continue solubilized. You can add as much solvent you want. You only Will have a solid Phase If you have more than 30 g in 1 liter of solvent.
By dilution you affect the accuracy of determination of solubility. The toxicity is given by the dose. You want to avoid larger volume in dosing.
Dear Sara shohdy Thabit dilution of such solutions is generally not a problem. If 30 mg of your compound is soluble in 1 ml of 100% ethanol or 100% DMSO it will also be soluble in 10 ml or 1 liter of the same solvents. On the other side, concentration of the solutions could cause problems if the maximum concentration is reached and the compound starts precipitating. The toxicity on living organisms depends more on the nature of the compound and the amount, but not on the concentration of the solution.
Good luck with your work!
SILAS DERENZO : Thanks a lot for your reply, actually I am going to dilute it with an aqueous buffer so that's why I am afraid it could precipitate.
Frank T. Edelmann: Thanks a lot for your reply, I am going to dilute with an aqueous buffer that's why I am afraid of the re-precipitation, I am afraid of the toxicity because high concentration of ethanol and DMSO are toxic on living organisms.
Dear Sara, thanks for your kind response and explanation. It was not clear from ypur original question that you want to dilute your drug solution in ethanol or DMSO with an aqueous buffer. Of course, in that case there is always the possibility of precipitation of the drug if it is more or less insoluble in water. In that case I would suggest to make a few quick dilution experiments with small amounts in small vials in order to practically test the limits of solubility.
Frank T. Edelmann : Thanks a lot for your reply, I am going to try this for sure.
Frank T Edelman is eight. It was not clear your goal and Aldo that It may or not precipitate when you make a dilution in a buffer. Since It behaves like a solvent with another composition. Experiments are necessary in this case, unless you find This dilution in literature. Good luck
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