I have synthesised curcuminoid-based compounds to test against my target protein. These compounds are highly hydrophobic and show poor solubility in aqueous buffers. I initially dissolved them in 100% DMSO, but upon dilution with buffer, the compounds precipitated, making them unsuitable for bioassays.
I tried adding Tween 80, and the solubility improved to some extent. Are there any other suitable strategies or additives to enhance aqueous solubility or prevent precipitation during dilution? Any suggestions are greatly appreciated
Improve curcuminoid solubility by using co-solvents (NMP, ethanol), cyclodextrins, surfactants, or nanoformulations to prevent precipitation. Amorphization and protein binding are advanced options for further solubility enhancement.
Abdelhak Maghchiche Hi Sir, your suggestion is highly appreciated. I tried dissolving my compounds in Tween 80 (0.1 %) non-ionic surfactant. Seems there is some good improvement in term of solubility in aqueous phase.
Polar solvents such as ethanol have high solubility for curcumin, which can effectively improve the solubility of curcumin. For curcumin, some polymers such as hydroxypropyl methylcellulose, polyvinyl pyrrolidone, etc. can be used as solid dispersants to disperse curcumin therein. Alternatively, a crystal transformation method can be used to transform a crystal form with lower solubility into a crystal form with higher solubility, thereby improving the solubility of curcumin.
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