XRF analysis of 45s5 analysis?

I've sent my synthesized 45s5 powder and a commercial 45s5 powder to XRF analysis but in both of them the amount of Na2O is about 15 percent and LOI is around 40 percent and it makes the aount of...

29 January 2021 239 4 View

Sol-gel synthesis of 45S5?

I've synthesized 45S5 bioglass by sol-gel method and my XRD and FTIR results are similar to 45S5 bioglass but my EDX result has differences and instead of Si and Na the amount of Ca is higher....

29 December 2020 4,847 2 View

Could anyone help me with this error Exception has occurred: ValueError?

I have downloaded monthly mean wind speed of terraclimate data for 20 years (2000-2019) which have NetCDF format, but unfortunately, each year has a single NetCDF file, and I want to merge them...

25 November 2020 8,477 2 View

Has anyone used Breaks For Additive Season and Trend for decomposing the time series into trend,and season in R package?

12 September 2020 1,513 3 View

How show NDVI MODIS index (MOD13A1) in Arc GIS?This collection of MODIS has calculated NDVI index and i want to show this index in my study area?

This collection of MODIS has 2 options for calculating NDVI index. First, we can calculate it from NDVI formula or we can use NDVI index that MODIS has calculated. I use both of them but they are...

25 August 2020 2,419 3 View

Has anyone used Maximum likelihood or Support vector machine (SVM) for image classification?

Hello I'm working on a project that I want to detect forest and deforestation areas. I need some information about Maximum-likelihood or Support vector machine (SVM) for image classification.

22 August 2020 1,896 3 View

How we can use the Transmisivity of light colors (rtn, gtn, and btn) in simulating a coated glass with red dyes in Radiance?

We want to simulate a red coated glass in Radiance. The UV-VIS spectroscopy analysis was conducted and optical characteristics of the red glass including transmittance and absorbance were...

16 March 2020 3,492 5 View

Model game as a discrete-time Markov chain?

Model game as a discrete-time Markov chain

30 December 2019 5,346 3 View

How can i remove fat from alkaloids?

sometimes i have problem with fat in my extracted alkaloids using ethanol and chloroform for extraction. Also my alkaloids are not well dissolved in acidic water, so acid-base precipitation...

09 December 2018 2,737 5 View

Is it wise to use EEG in order to analyze dynamic functional connectivity states?

Most of researchers are using fMRI for analyzing dynamic functional connectivity networks, I want to know if it is possible to use EEG as well and if so, what s the advantageous and...

11 September 2018 2,318 4 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View