Software of designing primers for RCA method (rolling circle amplification)

01 January 1970 1 6K Report

What are the suitable software of designing primers for RCA method (rolling circle amplification)? Is the software and designing rules different from primer design for PCR?

Dhuha Abdullah Kadhim

Rolling circle amplification (RCA) is a method of isothermal amplification of circular DNA molecules. RCA assay involves DNA amplification using a DNA ɸ polymerase with strand displacement activity to extend a single or multiple primers annealed to a circular DNA template

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