Soft agar experiment: I noticed overnight after plating upper layer, agar becomes thinner, almost liquid. Is not normal or not? Are ther techniques to prevent it? And bottom layer after 21 days become broken into fragments.
The Agar after plating is normally like a squishy gel similar to jam, but it never flows like a liquid.
Geometry Based Stochastic Channel Modelling or Spatial Channel Modeling (SCM) methods such as: - 3GPP-SCM, SCM-E - Winner (I and II) - QuaDRiGa produce channel coefficients as a matrix H (may on...
04 November 2020 9,480 2 View
This is so far the procedure I was trying upon and then I couldn't fix it As per my understanding here some definitions: - lexical frequencies, that is, the frequencies with which correspondences...
27 September 2020 4,742 3 View
Hi everyone, I want to learn as much as possible about survey methodology for evaluation purposes. I'm particularly interested in research around validity and reliability of: question types...
02 September 2020 413 10 View
Hello, I am looking for a way to compare the feature vectors of two images that contain a person in it. The application should be able to output a high similarity score for the same person and a...
10 May 2020 3,137 1 View
If you have to compare a variable among 4 groups (A, B, C and D -progressive disease stages) using a comparison test (student T test) between A and B, B and C, C and D is it necessary to perform a...
30 April 2020 9,742 5 View
We need to be closer to the nature and don't look for money and other values.
29 March 2020 1,238 4 View
Dear Colleagues, We are looking for an accurate relation between the magnitude of Cartesian octupole and Mie scattering coefficients. Something very similar to formulas (8) and (9) from...
19 February 2020 3,836 4 View
Hello, Is there a good robust way to isolate plasmids from some Actinobacteria which potentially possess them? Is it a combination of enzymes/sonication or something else?
26 May 2019 4,250 4 View
Out-of-sample validation (validation of model performance on an independent set of data) is a must in physical modeling, data mining, etc., but in a traditional statistics course it is not taught...
29 August 2018 5,514 4 View
19 May 2017 7,606 3 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
01 March 2021 2,027 2 View
I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...
01 March 2021 6,450 5 View
Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...
28 February 2021 7,292 3 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
20 February 2021 8,202 6 View
I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...
16 February 2021 169 4 View
I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...
09 February 2021 4,158 1 View
Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...
07 February 2021 1,671 2 View
03 February 2021 1,111 5 View
I want to simulate the tribological properties of solids, liquids, and fluids, please suggest good software.
02 February 2021 3,947 3 View