Does anyone have any suggestions on media, and ratio for smooth muscle cells and endothelial cells co culture?
Thanks!
Hello Mariana Perea
Have you referred to the paper attached below? Please refer to Table 1.
Article A system for the direct co-culture of endothelium on smooth ...
Proliferative media (PM) that stimulated SMC growth consisted of DMEM supplemented with 10% fetal bovine serum, 10% porcine serum, 0.05 g/L vitamin C, 3 × 10−6 g/L CuSO4, 0.05 m HEPES, 0.05 g/L proline, 0.05 g/L alanine, 0.02 g/L glycine, 10 × 10−9 g/L basic fibroblast growth factor, 10 × 10−9 g/L platelet-derived growth factor, and 1× antibiotic/antimycotic. To induce quiescence of SMCs, the quiescent media (QM) consisted of DMEM/F12 with 0.05 g/L vitamin C, 3 × 10−6 g/L CuSO4, 0.05 m HEPES, 0.05 g/L proline, 0.05 g/L alanine, 0.02 g/L glycine, 1 × insulin, transferrin, and selenium supplement, and 1 × antibiotic/antimycotic.
Prior to co-culture, ECs were grown in PM similar to SMCs except with lower serum content (5% FBS and 5% PS). Since ECs would not grow in the absence of serum, co-culture media consisted of 1 part of PM and 5 part of QM (3.3% serum).
You could use the ratio of EC to SMC cell densities ranging between 1:1 and 1:2 as per the article.
Best.
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