Cell lysis protocol for Picogreen DNA assay?

I'm trying to quantify the amount of DNA in adherent cells, does anyone have a good protocol for cell lysis to prepare samples for Picogreen DNA assay? Any help will be greatly appreciated! Gloria

04 May 2016 1,438 2 View

Can you measure amount of beta-tubulin protein with respect to beta-actin?

Both beta-actin and beta-tubulin are frequently used as load controls in WB, but can I measure the amount of beta tubulin in my sample and normalized with respect to beta-actin? In other words,...

04 May 2016 2,367 2 View

What type of brain cancer cell line is most vulnerable to T-cells?

I heard most brain cancer cells avoid t-cells very well, but is there a cell line (human or animal) that is a little more vulnerable to T-cells than others? thanks! Gloria

07 August 2015 446 3 View

How do you keep PC12 cells separate from each other?

PC12 cells easily form clumps, is there a way to prevent this clumping ? Thanks, Gloria

06 July 2015 6,563 2 View

How do you get subclonal of differentiated PC12 cells?

how do you get subclonal cell line of well differentiated PC12 cells? Thanks!

06 July 2015 1,542 2 View

Is there a way to transfer live cells, without freezing them for several hours?

Other than using a portable incubator, is there a way to transfer cells without freezing for many hours?

04 May 2014 3,444 14 View

What is a solvent for folic acid?

What is a solvent for folic acid?

04 May 2014 1,893 16 View

Can you get >100% cell viability in OD readings using MTT assays and other such assays?

I would like to know if you can get more than 100% cell viability using MTT assays. Thanks!

02 March 2014 308 6 View

Can anyone help with non-surface markers for THP1 cells?

What are good non-surface markers that I can use to characterize differentiated THP1 cells?

02 March 2014 6,977 3 View

What's good as a pre-coater for better platinum adhesion on SiO2

Any material good for enhancing platinum adhering on hydrophobic surfaces?

05 June 2013 7,999 2 View

How to calculate enzyme's energy of activation without rate constant (+excel)?

I want to calculate an enzyme's energy of activation, but I don't have the enzyme's molecular weight to calculate the rate constant or the pre-exponential factor A. This is part of an assignement...

25 December 2020 8,738 3 View

What is apparent Km Value of an Enzyme, Is it different from usual Km value and Why it is important?

It is very much confusing when some papers use this term "apparent Km of an enzyme even if they use single substrate ? Not at all clear ! Please help me understanding this. Thanks in advance.

27 September 2020 3,522 7 View

What is the difference between PCR and the initial steps (before SDS gel) of DNA sequencing? Which method is more effective?

I apologize if my question is not clear. I have a pure chemistry background and I am very new in a Protein Chemistry and Enzymology science.

25 September 2020 7,968 5 View

How to isolate/quantify a fungal enzyme for comparison of control vs. treatment?

I want to compare the amount of an enzyme's expression (laccase for example) or presence in the tissue as a result of the experimental treatment. Is there a protein similar to an antibody that...

31 December 2019 1,359 2 View

How strong is Flag tag binding to Anti-Flag antibody?

I am trying to plan an pulldown assay to find some prey protein for my bait protein. I am interested in using the flag tag because it is small and doesn't disturb structure and doesn't require...

11 December 2019 2,406 3 View

How to calculate the dosage of enzymes in wheat flour?

I have recently started working on improvement of wheat flour for baking. I have come across a research which involved the usage of xylanase, cellulase, beta-glucanase and carbohydrases for...

10 November 2019 3,608 1 View

Can I restrict the amount of one species when using KinTek for kinetic fitting?

I am using KinTek software to do a kinetic fitting. I have determined kinetics of the formations of several species in the time course of 5min and established a model for the enzyme (just as an...

09 October 2019 3,835 4 View

I am searching the pGex or pET plazid of HDAC6, if he is possibly for somebody and would give for me. Does somebody have this plasmid?

I am searching the pGex or pET plazid of HDAC6, if he is possibly for somebody and would give for me, would be needed for my work. I would like to test the interaction between my labor's new...

05 June 2019 4,133 1 View

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08 January 2019 3,512 5 View

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31 December 2018 8,083 5 View