Organic Rankine Cycle Simulation?

Am working on my thesis involving organic rankine cycle (ORC) modelling. I aimed at using simulink in building the model, but am finding it difficult to assemble blocks in Simulink that would give...

22 January 2020 2,135 3 View

Studying boundary value problem, is it reasonable to think of first order ODE?

I am very familiar with problems of the form y'(x) = f(r; x, y), y(a)=y0, y(b)=y1 where r is a parameter to be estimated using the boundary values. But apart from this situation, has anyone...

23 October 2019 2,864 3 View

I have a data set and I will like to use pooled cohort equation to estimate the risk for ASCVD. What software can I use to this?

I want to estimate the 10-year risk for atherosclerotic cardiovascular risk for each of the 2500 participants in a study. Is there a software I can use to do this at once?

02 October 2019 4,629 1 View

Intrusion Prevention Systems?

I am planning to do a dissertation on intrusion prevention system. Can anyone give suggestion on how to develop one from scratch? Like what are the things that I should look upon. I have a fair...

27 May 2019 8,861 10 View

What is the possible growth mechanism (other than Diffusion and shadowing) that could possibly result in branching of titanium nanocolumns?

Through ebeam GLAD, i was able to deposit titanium nanoparticles at subtrate temperature 260C and room temperature. The issue here is that while analysing the morphologies of the nanoparticles...

16 April 2019 370 3 View

Instruments for measuring Entrepreneurial Ecosystems?

I am working on entrepreneurial ecosystem and I need instruments to measure it.

01 January 1970 7,709 2 View

Intrusion Prevention Systems

I am planning to do a dissertation on intrusion prevention system. Can anyone give suggestion on how to develop one from scratch? Like what are the things that I should look upon. I have a fair...

01 January 1970 7,450 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View