How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Why the presence of surface active compound repress the mass spectra of other compound? If the sample is itself surface active, then self repression?

I am assuming the resulting insertion/agglomeration in the presence of a surfactant or the agglomeration of surfactant may reduce the rate of ionization. The mobility of the ion may also be...

07 January 2021 987 5 View

Can a compound have LogP value around 0.05 and LogD value around 4.02?

I understand that there might be huge differences between LogD and LogP for ionizable compounds. Normally, logD should be lower than logP for ionizable compounds. But can LogD value be...

05 January 2021 6,714 0 View

Do I really need matrix matched solutions (ICP-OES)?

Acording to my knowledge I know I should have matrix matched blanks, standard solutions and samples. It's really hard if you have large variety of digested samples. Standard addition is also time...

03 December 2020 9,783 2 View

Under which circumstances can drug SALT solubility in water be converted into drug INTRINSIC solubility?

Most drugs are ionizable in aqueous solutions: their solubility is pH-dependent. Ideally, prediction of solubility of new drug entities involves training sets consisting of INTRINSIC solubility,...

17 November 2020 2,650 2 View

How can i make a 1000 ppm Ni(II) solution?

I try to prepare 100ml of a 1000 ppm Ni(II) solution by solving NiSO4.6H2O in ionized water. To do so I add 0.45g of NiSo4.6H2O to the water. i would appreciate if anyone tell me whether it is...

31 August 2020 7,807 4 View

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home?

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home (freeware)? Chromatograms were made by Waters UPLC I-Class PDA-MS. At the workplace,...

14 May 2020 5,383 3 View

Short Peptide synthesis, Fmoc-SPPS wth citrullin, why can it show a different mass?

I tried Fmoc-SPPS with short cyclicPeptides (12 aa length, Brom-Acetyl at the start for cyclization), for the first time containing citrollin. Many of the petides show a main Peak at the MALDI MS...

31 March 2020 3,986 3 View

Where are protons in ESI+ formed in LC-MS/MS?

Can anyone help me with peer reviewed papers that explain whether or not ions are formed in the ESI source? Is this what they call 'right-way-round' principle? Then how come that some for some...

10 March 2020 2,495 1 View

How can I measure concentration of a gas (for example ammonia gas, H2S or Carbon monoxide) in air?

I need to measure gas concentration in a system before and after filtering. But I don't have access to some of these gases sensors and I don't have enough information about available analysis...

23 January 2020 3,535 3 View

Silver Copper Ionization Disinfection or UV?

Which among these would be effective water disinfection method? What are the possible disadvantages of UV water disinfection? Can the technology of Cu-Ag disinfection come up in future? What are...

31 December 2019 9,280 4 View