The paper below knocks out both allele of B2M gene using adenovirus.
https://www.sciencedirect.com/science/article/pii/S152500161632617X
They have targeted B2M allele by transducting adenovirus sequentially using two different antibiotic selection gene. Why do they have to target it twice? idelaly, single transduction should work since homologous region are same in both allele. If I have to do homologous recombination for a particular gene, do I have to generate two virus with different selection gene? Why should single transduction work? I need an urgent help of scientific community.
Thanks,
Mohit
Hi ALex,
Thanks for your comments on my question. DO you have prior experience with Adenovirus? If we leave apart what is being said in this paper, and from your experience, do you think single DNA sense strand containing adeno will replace the target locus at both the allele of a gene, I mean I would get homologous recombination? I have also read that adenovirus targets only one allele of a gene (Addgene's page). Any thoughts what this might mean?
Thanks,
Mohit
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