Hi there!
I was hoping that someone who works/ed with Aqualog Horiba (with polarizers) could assist me in this matter. Currently I am developing a methodology to analyse protein-polymer (nanomolar range) interaction using EEM.
During the experiments, even applying different equipment settings and sample conditions, there is a sensible change on light scattering (+-30% on intensity) whereas emission is fairly constant (error +- 5%).
For these experiments I'm using 10x2 mm quartz cuvettes freshly cleaned and using the same sample per cuvette, avoiding other external changes that would impact our results.
In addition, these samples are summited to light scattering analysis (DLS) and there is no correlation between scattering and size. Anyone had similar issues?
The HORIBA Aqualog is a unique optical spectrometer that is the gold standard in environmental water research around the world for the study of color dissolved organic matter (CDOM).
Please use a scanning fluorometer for EEMs, but a much faster and better A-TEEM spectrometer for colored dissolved organic matter (CDOM)
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