Predicting Depression Levels Using Social Media Posts

The use of Social Network Sites (SNS) is increasing nowadays especially by the younger generations. The availability of SNS allows users to express their interests, feelings and share daily...

01 January 1970 4,400 2 View

What is the role of asparagine in cancer?

Asparaginase is useful in the treatment of leukemia as it degrades asparagine. What role does asparagine play in the proliferation of cancer cells?

01 January 1970 4,720 3 View

Impact of the Covid-19 pandemic on the Aquaculture and Fisheries sector.

Hello all, With all the lockdown regulations, sales and trade may not be as it was before, goods and services are impacted; fish and fish products and services are not left out. Would you...

01 January 1970 3,700 13 View

The Role of Renin-Angiotensin-Aldosterone System in the Severity of COVID-19 in Diabetic/Hypertensive Patients

Hypertension, diabetes, and cardiovascular disease are the most prevalent comorbidities in patients with COVID-19. Although they do not appear to affect the infectivity of the virus, they do...

01 January 1970 8,859 8 View

The 'Biochemistry' of permed hair

Is there more to a permed hair than the alteration of the disulphide bonds between adjacent strands of protein in hair?

01 January 1970 8,589 3 View

'Biotechnology'- A textbook authored by V. Satyanarayana

Please who can help with copy of this textbook in PDF?

01 January 1970 5,980 5 View

Cancer cells and metastatic phenotype

Why do cancer cells use distinct transcriptomic and proteomic programs to reach the same metastatic phenotype?

01 January 1970 4,948 3 View

Biotechnology: Do the Benefits Outweigh the Downsides?

The major apprehension of genetic engineering is that through recombinant DNA experiments , unique micro-organisms or viruses (either inadvertently, or sometimes deliberately for the purpose of...

01 January 1970 965 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View