How can I make a preparation of 1M pH 6 potassium dihydrogen phosphate buffer?

I can't seem to find the answer online or in my lab anywhere.

09 February 2020 8,490 3 View

How do you lyse small cells (10 microns) without disrupting the mitochondria?

We consistently isolate intact/functional mitochondria from liver and heart tissue using manual homogenization (to disrupt the cell membrane) followed by differential centrifugation. I am now...

04 November 2019 9,279 1 View

In your research with OrganoTropisms are you utilizing tissue culture or histology slides?

Tropisms for various organ systems by borreliae have intrigued me for many years. In my research I have utilized autopsy tissues and tissue cultures for examination with Immunohistochemistry and...

17 March 2019 773 1 View

How do hallucinogens work on the brain?

Robin Carhart-Harris, Mendel Kaelen and David Nutt consider a big questionon several levelsDoes self-organised activity in thedefault mode network underlie oursense of self or ego?

07 August 2016 7,296 0 View

Why are we conscious?

07 August 2016 5,818 2 View

What system mediates and incorporates all the potentially competing signalling of the brain?

Does the brain work in harmony, for example in a way where signals work up a chain of command and through the way the modularities of the connectome are integrated they are all given a proportion...

07 August 2016 4,341 0 View

The processing power of the brain, way-beyond binary; Could a Turing like machine decode the encryptions of the human brain?

We clearly process information, the neurochemical signatures are a way neuronal communication occurs between modulars of the brain. We have at least 50 neuro communication chemicals, that is...

07 August 2016 4,129 0 View

How can one make a dilution series before determining titer?

11 March 2015 7,875 3 View

Do you remove the Peyer's patches when doing FACS analysis on the mouse colon or include them in the analysis?

I am looking at the impact on the immune system with different treatments. Should I include the Peyer's patches when putting cells through flow cytometry? Or should they be removed and ran separately?

09 September 2014 8,874 5 View

A placebo-controlled investigation of synaesthesia- like experiences under LSD?

01 January 1970 8,512 4 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Which are the journals for free paper publication?

A bunch of flower for everybody. I would like to ask about the list of journals which we can publish our paper for free. Thanks in advance. Sincerely Walid

25 February 2021 9,790 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Hello, does any one know a free medical text dataset?

a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards

22 February 2021 5,988 2 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Can anyone recommend which is the best free questionnaire software please?

Hello, I am looking to send out links to an online survey. My previous research project used a paid for survey software. I am now looking for something that's free but straight forward to...

19 February 2021 5,641 3 View