How we can dissolve theophylline derivatives for the MTT test?

I have a question about your letter, Fluorescent phosphoinositide 3-kinase inhibitors suitable for monitoring of intracellular distribution. Can you tell me how we can dissolve theophylline for...

26 January 2021 9,094 1 View

Can anybody suggest me a cheap material for surface modification of hydrophilic Titania nanoparticles?

I'm looking for a cheap material to modify Titania nanoparticles in order to use them in polymer and increase its contact angle.

13 December 2020 504 5 View

Importance of the tunnel effect on the solar cells performance?

Does the tunnel effect play a major role in the performance of solar cells? Especially when the device is heavily doped so the depletion layer can be thin enough for tunneling. Are there any...

10 December 2020 5,518 10 View

How can I find a questionnaire for Corporate identity?

My thesis is about the relation between corporate social responsibility and corporate identity. And I can not find any questionnaire about it. Can you help me?

24 July 2020 3,507 3 View

How to deal with a skewed Likert scale data with Ceiling/Flooring effects in a before-after design?

I have data of 30 subjects from a before-after design study. We used likert scale to investigate effects of changes in mood on some dependent variables. We hypothesized that the intervention would...

22 February 2020 6,496 5 View

What are the quantities per microliter of the different components needed for a reaction (1isolate of S. aureus ) to run a multiplex PCR ?

Dear colleagues, I need help. I want to run a multiplex PCR to look for enterotoxin-encoding genes in coagulase-positive StaphyloccIoccu . I have all the reagents needed to prepare the MIx PCR...

13 February 2020 7,036 2 View

Which international universities hold the Digital Transformation DBA course?

Hi dears, I want to learn more about Digital Transformation and its impact on Business model, strategy, marketing, agility and ... .I want to apply for online course at universities; is there any...

24 December 2019 3,817 11 View

I performed P-AKT western blot experiment. Even in 0 MOI, I got band. Is there any suggestion?

In any other phosphorylated proteins, in 0 MOI, I did not get band, but I got band in P-TAK.

22 July 2019 1,890 5 View

Removing a column which entirely has Zero value?

Hi! I have a very big excel sheet (6000 (row)*16000(column)), in which in some of them the entire columns have zero value. Does anybody knows how to remove those columns (just not manually)?

18 February 2019 8,960 3 View

How to count cells of a plant tissue in mitosis manually without using a cell counter ?

Count cells of plant tissue

15 February 2019 4,563 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Is that possible to do an impedance measurement without FRA module?

One of my colleagues said, "the impedance you can measure with the three electrodes and with the specific sequence varying the frequency" Is it possible to configure the potenciostat without the...

21 February 2021 418 2 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

How to show in a scientific paper format the post-hoc test of 4 variables involved, when 3 by 3 variables are significant?

In a study involving 4 variables (species(2x), temperature(2x), nutrients(2x) and light(3x)), 3 by 3 variables, for example, "species", "temperature" and "nutrients" are significant. I end up with...

15 February 2021 8,018 2 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

Why do my polyphenol measurements fail?

In order to measure the polyphenols in broccoli, we prepared a reagent for measurement using the following procedure. 1. Extract the crushed plant with 70% methanol. 2. Mix 1 ml of the extract...

07 February 2021 845 4 View

How to mesh complex geometry?

I have been trying to mesh some complex geometry and have been having issues. I have attached images of the geometry in question. This structure is very large in size but has some comparatively...

05 February 2021 6,379 5 View

How to calculate Total Phenolic Content and express it in mg GAE/kg?

26 January 2021 3,847 7 View