I did extraction of plant and i got crude extraction for petroleum ether, Chloroform, and methanol and I want to check the antibacterial activity for these three extractions. should I prepare different concentrations of solution that used for extraction for checking in a same solvent that I used for extraction or there is another solvent less toxic for that?
prepare different concentrations of solution that used for extraction
Evaporate the solvent and take the dry sample up in a water-miscible, nonvolatile solvent that is compatible with biological testing. The usual choice is dimethylsulfoxide (DMSO). (You could also try water, but it is unlikely to dissolve much of the material that was extracted with nonpolar solvents.) Most bacteria can tolerate a few % of DMSO by volume.
There are specific guidelines for dissolving each antibiotic for susceptibility testing in clinical microbiology labs, which are available (for a price) from the Clinical Laboratory Standards Institute (CLSI). However, for research purposes, I would suggest using the same solvent for all the drugs in your study, as long as they are all soluble in it. DMSO works for most drugs.
I did extraction of plant and i got crude extraction for petroleum ether, Chloroform, and methanol and I want to check the antibacterial activity for these three extractions, I used DMSO to dissolve the crude drug but for PE and CHCl3 crude extractions not dissolved completely in DMSO any anther solvent suggest Thank you in Advance. Adam B Shapiro Ali Safaa Muazzam Sheriff Maqbul
Since the solvents chloroform and petroleum ether are very hydrophobic, the substances they extracted from the plant are also very hydrophobic. It is not surprising, therefore, that these substances do not dissolve well in a polar solvent such as DMSO. I doubt that it will be possible to dissolve these substances in any solvent that is compatible with the broth microdilution assay. You could try adding the organic solution to a filter paper disk and letting the solvent evaporate, then doing a disk diffusion assay on agar, just in case something in the extract is able to diffuse through the agar.
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