should i use 1 octane sulphonic acid instead of TFA(triflouroacetic Acid) in HPLC reverse phase as both are ion pairing agents?
The trouble with ion pairing agents is/are that they coat the column (stationary phase), thus permanently changing the column's selectivity (a C18 column is no longer a C18 column). Thus, once an ion pairing agent is used the column has to be dedicated for that particular separation.
I would avoid 'ion pairing agents' at ALL costs! If you are not getting RP-LC retention try a more hydrophobic phenyl phase and then try HILIC (if the molecule is particularly water loving).
The trouble with ion pairing agents is/are that they coat the column (stationary phase), thus permanently changing the column's selectivity (a C18 column is no longer a C18 column). Thus, once an ion pairing agent is used the column has to be dedicated for that particular separation.
Ion pairing reagents are NOT recommended as they cover the st. phase of the column and reduces retention selectivity
Got the answer but TFA in small concentration is also used as ion pairing agent..its only 0.1% TFA and i want to use the same one octane sulphonic acid.
TFA is a much more respectful chemical towards your column. I would avoid
With the newer, highly-polar columns and HILIC columns available there really isn't a good reason to run ion-pairing reagents. None of them are MS friendly, so if you ever want to move your method to LC-MS you'd need to eliminate them anyway. I'd look at changing columns before I used octanesulfonic acid.
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