I am new to IHC. I looked over several protocols for IHC of tyrosine hydroxylase (TH) and saw that some use 1XPBS, some use 0.1M PBS (10X), or PBS+Triton X-100, or TBS, for rinsing sections and diluting antibodies. I am not sure it is simply personal preference or is there benefit to using a particular over over the others. Could someone please tell me which one I should use? Thanks in advance.
Hi Ying,
You can use 1X PBS without any issues. But for first immediate wash after Ab incubation, using 1XPBS with 0.05-0.1% Triton X-100 may give you better images in terms of the background. You may dilute your Ab in 1XPBS containing 1%BSA.
For TH, if you are using immunoperoxidase through DAB, then use 0.01 PBS with 0.3% Tx..look at this paper
Article Admixing of MPTP-Resistant and Susceptible Mice Strains Augm...
all the best
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