I will using tetra-primer ARMS PCR for genotyping specific SNP in adiponectin gene. I have taken the  4 primers designed in a previous paper. but I noticed that the paper used one annealing temp for the 4 primer in one pcr reaction. I don't know will this give accurate result or not. I want to know is it better to use 2 annealing temp (one for outer primers and one for inner primers) but I don't want how many cycles for the Ta of outer primers and how many for Ta of inner primers