Should I calculate dose for each rat in each group?

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Peter Owira

You have not indicated the route and vehicle of dosing. Is it via oral gavage? Yes you should dose according the weight of each rat since the rats will not be of the same weight. A rat weighing 300 g will get 50/3 mg. Make a stock solution of the test compound and if dosing by oral gavage then you can only vary the volume of the solution administered according to weight of the rat.

Fatma Mohamed Fouad

Peter Owira thanks. its by oral way