Fatma Mohamed Fouad

13 Questions 18 Answers 0 Followers

Questions related from Fatma Mohamed Fouad

Should I calculate dose for each rat in each group?

I am new in dealing with rats experiments. I have 7 rats in each group and I want to give them 50mg/kg dose but they are different in weights, should I calculate the number of grams from my drug...

4 weeks ago 127 2 View

What is the range of cell density for cell proliferation assay ?

I want to treat cancer cell lines with a treatment, and I want to calculate IC50. Should my cells after 24 hr of seeding be confluent or what is the suitable percentage that cells should be?

12 August 2025 6,342 7 View

What is the optimum optical density of ovarian cancer cell lines for cell proliferation assay?

I am working with two cell lines and mostly I got 0.5 OD in control cells even when I increase the cell numbers and I am using Alamar blue assay for 3 hours, is that ok or I should get higher...

10 July 2025 8,122 2 View

How do I create serial dilutions of a pure compound (that was dissolved in 100%DMSO) while maintaining a 0.1% DMSO concentration in each solution?

I need to test various concentrations of a compound that is only soluble in 100%DMSO. My compound is in pure powder form (I have 1g available, and I will dissolve in 100% DMSO to prepare my...

01 June 2025 4,775 14 View

Can I used log2 fold change in graph of each gene in qPCR results instead of relative exoression?

I am validating genes according to transcriptome data using qPCR, I want to represent all validated genes but I need each gene represented in its own graph. I only did two replicates of each...

20 October 2024 6,199 0 View

How get ride of high error bar?

I am working in transcriptome analysis to validate differentially expressed genes in cell lines treated with two different treatments and I choose some genes to validate by qPCR but when I tried...

25 September 2024 1,626 3 View

Can I use MTT assay for several time?

I am new to using MTT assay. My experiment is to treat cells and see the cell viability after 24,48,72 hrs so should I grow cells in three different plates and put MTT reagent for each one...

10 March 2024 6,540 4 View

Can the color of media with agrose used for soft agar assay be changed ?

I did soft agar assay for MDA_MB_231 cell lines, but after 3 days the color of agrose changed to yellow. Is that ok or there is a problem?

01 February 2024 4,436 0 View

How can I re-incubate the PVDF membrane with new antibody?

For the western Blot, I need to check another antibody in my membrane but this antibody is from the same species as the first antibody so how can I do that without stripping? (Note: two antibodies...

15 December 2023 6,342 4 View

Can RNA be stored with isopropanol ?

Can I store RNA in isopropanol for a long time at -20 °C?

29 October 2023 3,123 2 View

Why each two positions near to each other ?

In a research paper, they mentioned this phrase "We have noted two numbers of mutation in some positions, which are nucleotide positions at 39,139, 44,220, 61,844, 63,147, 77,807, 132,625,...

29 May 2023 6,306 3 View

I need to identify if this if fungi or mold or not?

It was isolated from algae in the sea, it was grown on PDA media with lactic acid

04 May 2023 1,370 2 View

What is the suitable solvent for all types of proteins?

I want to do a MALDI-TOF analysis for total protein extracted from rice, what suitable solvent can I use?

25 February 2023 3,807 4 View