Fatma Mohamed Fouad

7 Questions 8 Answers 0 Followers

Questions related from Fatma Mohamed Fouad

Can I use MTT assay for several time?

I am new to using MTT assay. My experiment is to treat cells and see the cell viability after 24,48,72 hrs so should I grow cells in three different plates and put MTT reagent for each one...

10 March 2024 6,457 4 View

Can the color of media with agrose used for soft agar assay be changed ?

I did soft agar assay for MDA_MB_231 cell lines, but after 3 days the color of agrose changed to yellow. Is that ok or there is a problem?

01 February 2024 4,388 0 View

How can I re-incubate the PVDF membrane with new antibody?

For the western Blot, I need to check another antibody in my membrane but this antibody is from the same species as the first antibody so how can I do that without stripping? (Note: two antibodies...

15 December 2023 6,287 4 View

Can RNA be stored with isopropanol ?

Can I store RNA in isopropanol for a long time at -20 °C?

29 October 2023 3,060 2 View

Why each two positions near to each other ?

In a research paper, they mentioned this phrase "We have noted two numbers of mutation in some positions, which are nucleotide positions at 39,139, 44,220, 61,844, 63,147, 77,807, 132,625,...

29 May 2023 6,256 3 View

I need to identify if this if fungi or mold or not?

It was isolated from algae in the sea, it was grown on PDA media with lactic acid

04 May 2023 1,325 2 View

What is the suitable solvent for all types of proteins?

I want to do a MALDI-TOF analysis for total protein extracted from rice, what suitable solvent can I use?

25 February 2023 3,777 4 View