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Questions related from Fatma Mohamed Fouad
I want to treat cancer cell lines with a treatment, and I want to calculate IC50. Should my cells after 24 hr of seeding be confluent or what is the suitable percentage that cells should be?
last month 6,333 7 View
I need to test various concentrations of a compound that is only soluble in 100%DMSO. My compound is in pure powder form (I have 1g available, and I will dissolve in 100% DMSO to prepare my...
01 June 2025 4,767 14 View
I am validating genes according to transcriptome data using qPCR, I want to represent all validated genes but I need each gene represented in its own graph. I only did two replicates of each...
20 October 2024 6,194 0 View
I am new to using MTT assay. My experiment is to treat cells and see the cell viability after 24,48,72 hrs so should I grow cells in three different plates and put MTT reagent for each one...
10 March 2024 6,534 4 View
I did soft agar assay for MDA_MB_231 cell lines, but after 3 days the color of agrose changed to yellow. Is that ok or there is a problem?
01 February 2024 4,427 0 View
For the western Blot, I need to check another antibody in my membrane but this antibody is from the same species as the first antibody so how can I do that without stripping? (Note: two antibodies...
15 December 2023 6,328 4 View
Can I store RNA in isopropanol for a long time at -20 °C?
29 October 2023 3,110 2 View
In a research paper, they mentioned this phrase "We have noted two numbers of mutation in some positions, which are nucleotide positions at 39,139, 44,220, 61,844, 63,147, 77,807, 132,625,...
29 May 2023 6,293 3 View
It was isolated from algae in the sea, it was grown on PDA media with lactic acid
04 May 2023 1,362 2 View
I want to do a MALDI-TOF analysis for total protein extracted from rice, what suitable solvent can I use?
25 February 2023 3,800 4 View
