I am interested in synthesizing AgNPs from plants with potent anti-cancer activity, so should i synthesized NPs from aqueous extract directly or screen the extract from d/f solvents for in-vitro anti-oxidant activity and then go for synthesis from the active extract? I have the same question for anti-microbial, ant-fungal and anti-leshminial activity. Thanks
Dear Mr. Ovais
Most of the researcher used the aqueous extract of plant to prepare the Ag-NPs. And it might be different fractions (i.e. extracted by different solvent) have different capabilitytowards silver reduction just due to the presence of variety/classes of phytochemical. So the morphology properties (i.e. size, shape, zeta potential of Ag-NPs) might be change, and this ultimately affect the antimicrobial, antifungal and antileshminial activity. You can try the aqueous and some other solvent extract to prepare the Ag-NPs and characterized them to see the change in the morphological properties of prepared Ag-NPs.
Thanks alot Anand Baraparate for your valuable answer, one of another friend from india "Sudip Mukherjee" has answered this question as following.
"It depends upon the presence of the anticancer molecule in the corresponding extract. Check the available literature regarding the plant and do either water or MeOH extract.
regarding antibacterial property, AgNPs has its own activity against bacteria, so no need to bother about the medicinal active phytochemicals"
I personally believe that the part we are taking for NPs synthesis should be properly first correlated with the application we are applying, if we are going for activities of AgNPs as anti-cancer agents then obviously that part should be selected which is enriched in particular anti-cancer compounds (From literature we can get this info) same goes for exploiting nanoparticles as contrast agents and if we are going for applications like making Ultrasound gels, water purification, drug delivery agent and anti-microbial activities then any plant part capable of reducing Ag+ will do the job.
Dear Muhammed Ovais
We used aqueous extract to synthesize AgNPs. I think aqueous extract will be enough for further in-vitro studies.
Further, the antimicrobial properties of silver nanoparticles depend on:
1. Size and environmental conditions (size, pH, ionic strength).
2. Capping agent
Thanks Chandra for your valuable answer. Definitely in most of the cases aqueous is preferred.
Dear you can use aqueous extract directly as most of the researcher proposed for you