In the case of the multidrug resistance transporter Mdr1 and its antibody C219 the data can be fitted by a Sachs-Welt random-bi model (10.1046/j.1432-1327.1999.00643.x). The binding curves for different models are quite different, simply look for the least complex model that gives a decent fit, i.e., the deviation of the data from the model can be explained by their standard deviation. There is an F-test for that.

Huiwen Wu

Use 1:1 Langmuir model of binding to calculate the affinity of Abs.

Hi,

1:1 binding model is best suited for bivalent interactions ( i.e. antigen-antibody). For more details about this binding model you can read this research article

Article Determination of High-affinity Antibody-antigen Binding Kine...