Any good protocol and procedure method to make a successful HRM experiment in A.thaliana? I have to verify SNPs i already know in two population of plants to check genotype after KASP procedure.
I need the contacts of a company able to furnish or support the set up of a RUSITEC, a continuous cell rumen model. Could do you be able in supporting me? Or, at least, do you have a project of...
25 January 2022 6,115 4 View
I have been asked to use the 284.8eV peak in the C1s narrow scan as the reference peak for fitting all other potential peaks for a carbon material. Also, which fitting optimization parameters...
11 January 2022 6,308 5 View
I tried different protocols but at the beginning of the procedure the tissue (starfish gonads) gelifies and blocks the other extraction phases.
24 May 2021 3,511 1 View
Hi All, I am carrying out an international research with clinicians (speech-language pathologists/ therapists). International professional associations will be distributing the questionnaire link...
02 February 2021 5,577 3 View
About 10 years ago, I experienced that the black leaf clips used with Pocket-PEA fluorometer (firmly suggested by Hansatech vs. the white version used for Handy-PEA), when used under high...
31 July 2020 5,853 0 View
I have some problem with baseline of my DAD (G1315B Agilent Technologies). I have tried to wash the cell (bypassing column) with isopropanol for a night. The issue seemed to be solved, but during...
10 December 2019 647 0 View
Hi All, I am currently undertaking a systematic review. 19 studies were classified as studies awaiting classification. I have contacted the authors. Several authors did not reply and a number...
22 July 2019 4,213 1 View
Hi All, I am new to the field of sEMG so any guidance or feedback would be greatly appreciated. I am collecting facial EMG data from two groups: healthy vs individuals with neurological impaired...
19 June 2019 6,178 3 View
Hi All, I am currently undertaking a systematic review. Two of the studies, completed by nearly the same authors, seem to have used the same set of participants. The differences mainly lies in...
30 May 2019 7,209 8 View
I am looking for a way to quantify autophagosome maturation before fusion with lysosomes to see if there is any defect in cargo recognition. I've read about co-localization studies of p62 and LC3...
26 May 2019 2,503 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View