Hello,

I would like to ask about your experience with the assessment of serum Glypican-3 by ELISA. We have used reagent kits from two different manufacturers and got no results. In the first case, already the standard curve was with many errors (lower concentrations in higher standards). When performing the second analysis, standard curve was ok but all serum samples concentrations were bellow the blank value, and the spiked ones only a bit higher. The plates were prepared by an experienced lab assisstant. Do you have any clue? Thanks a lot!