Separation of inner and outer membrane in bacteria?

24 December 2018 1 3K Report

Hello everyone and just a quick question for better understanding: when I separate the inner and outer membrane of bacteria using a sucrose gradient, the outer membrane fraction is below the inner membrane fraction. Question is are they separated based on the 'weight' of each of the membrane fractions or is this separation a result of the different composition of the membranes?

Thank you in advance and all of you calm and peacefull christmas holidays.

Best regards,

Erik

Yoram Gerchman

Separation in gradient is never based on "weight" (if it was ships would have sink rather then float). It is always based on density - and this is based on composition.

Take a look here

Article Separation of Inner and Outer Membrane Vesicles from Escheri...

Badges
Science topic

More Erik Lehmann's questions See All

ELISPOT normalization with varying cell numbers?

Hello everyone, due to a calculation error in the spreadsheet, the splenocytes I used were wrongly diluted. As a result, the number of cells per well are identical for each mouse, but differ...

15 December 2020 9,344 1 View

How do I structure my panel data in an event study?

Hey, I am conducting an event study with a short event window (lets say 3 or 5 days). I have a lot of variables, but most of them are fixed over that short period of time (GDP per capita, GDP...

11 November 2020 3,582 6 View

Jamovi - mean centred moderators?

Jamovi free ware has a built-in interaction function (a product term) in linear regression procedure. Does anybody know if Jamovi automatically mean centres variables included in the product...

04 August 2020 6,418 8 View

Supplier for thick block of brass CuZn20?

For a ballistic test I am looking for a supplier of brass CuZn20 in thick plate or billet shape We need 150 mm in total thickness with sides (or diameter of 160 mm).

17 May 2020 449 4 View

How to extract legal speed limit from GPS coordinates in OSM (OpenStreetMap) ?

I am interested in the extration of speed limit from Longitude and Latitude coordinate values. Is it possible to find speed limit attribute assigned to specific Open Street Map position and is...

30 March 2020 9,201 3 View

Human Isogenic H3.3K27M cell lines?

Hello all, I'm trying to identify pairs of isogenic human cell lines (preferably brain) in which the parent line does not possess the H3.3K27M mutation while the other one does. I've been finding...

26 March 2020 1,439 2 View

Has GPS detected the ethrer bwind?

Abstract An illusion of undecidability of an ether wind was the basis for establishing the theory of special relativity (SRT). The reason to that mistake was a wrong interpretation of light...

08 February 2020 6,990 4 View

Does source code language matter in a paper?

During literature review, I have noticed that many papers under the umbrella of software engineering use Java when code examples or small demonstrations are appropriate. I understand that Java is...

10 January 2020 1,763 7 View

How do we measure the concentration of two different complex in the same solution: Rh2(SO4)3 and RhCl3?

Hi all. I have solution containing mixture of two complexes of rhodium Rh2(SO4)3 and RhCl3, the concentration about 100 ppb - 2 ppm each with matrix SO4 2- and Cl- about 2 M, is there any...

11 November 2019 5,753 1 View

Does anyone know a reliable dataset of wave height in different locations of the Mediterranean Sea?

14 May 2019 6,318 4 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View