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30 August 2020 2,230 5 View

How to get pure recombinant virus with plaque purification ?

We are working on getting pure desired gene transfected virus. We used YFP fluorescences tags to distinguish the virus with or without gene replaced. Using PCR testing, we always have wild type...

01 August 2020 2,087 1 View

How to make Sentaurus silver.par to be the perfect conductor like defined electrode?

I have been trying to create some perfect conductor in my simulation as a connection between 2 spots(different doping semiconductor area). But is seems that the barrier bewteen silver and the...

10 March 2020 2,535 1 View

Do anyone have the experience of testing creatinine (kidney function) by using HPLC?

In my experiment, I want to use serum (mouse) to test the creatinine level via HPLC. 1. During the preparation, I centrifuged the whole blood, and then transfer the supernatant (which is serum)...

17 February 2020 9,679 2 View

What is the best indoor spectrum that people need?

What is the best spectrum for an office, classroom, or other architectural scenes? Spectrum affects people's visual needs, work efficiency, comfort, psychological or physiological rhythm. How to...

20 December 2019 5,715 4 View

What effect does precipitation pH and heat treatment temperature have on the electrical resistance of the material?

I have prepared a series of nanomaterials by co-precipitation under different pH value and heat treatment temperature. I want to find a reasonable explanation about the change of the material...

21 August 2019 9,192 3 View

Sentaurus simulating anti-reflection coating issue?

I am starting to simulate the anti-reflection coating (ARC) for solar cells but I found some problems that I can't answer: 1, For solar cell without ARC, I tried to put the light source below...

03 July 2019 6,682 2 View

How to consider the question of theoretical support for statistical model choosing?

In my research I focus in paired correlated binary data. To accommodate the intraclass correlation, I choose to adopt a certain model without providing much theoretical support, and was criticized...

04 June 2019 7,887 12 View

does anybody have DR5::GUS vector to share?

does anybody have DR5::GUS vector to share? i need to check auxin in potato. thanks a lot.

03 March 2019 3,449 6 View

Why the ZnO I grinded photodegrade slower than bulk?

These days, I do dye degradation experiment. I use mortar and pestle to grind bulk ZnO 10 min, the powders turn yellow. Then use that powder to do dye degradation, why it degrades slower than...

05 August 2018 1,769 4 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View