How to get a quantitative phase analysis from small amounts of sample?

Hello, Due to project internal reasons I can only obtain samples (multi-oxide systems) having a volume being to small for a quantitave XRD analysis. Is it correct to fill up the sample with a...

15 January 2021 8,389 5 View

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I am looking for high purity calcium phosphate powders. In order to fulfill a special application, it is necessary to obtain sintered bulks being white, but not blue. can anybody help me? Thank...

10 May 2020 1,295 3 View

Is there a free or low cost software for editing chromatograms?

For example I want to compare, align and edit forward and reverse chromatograms (by changing, deleting, inserting bases etc. within the chromatograms) and finally get the consensus sequence in...

26 June 2019 7,103 3 View

Protocols for determining live cells grown on transwell filters? Dye? LDH?

11 October 2016 4,436 3 View

Any differences in ecology and distribution Eleusine indica and E. Eleusine tristachya in Europe?

hallo, I need some information about the occurrence and distribution of Eleusine in (South-) Europe (Mediterranean, Spain/Iberia) incl. Canary Islands. Are there distribution maps? What are the...

30 October 2014 3,447 5 View

Has anybody the equipment for annealing a sample under a H2/CO2 atmosphere?

06 August 2014 2,525 2 View

Selection of MSCs/ECFCs with puromycin after transduction - can anyone help?

12 March 2014 663 1 View

How does the distance between the layers in bilayer graphene vary with perpendicular pressure?

I wanted to calculate the change in distance "d" between the two layers in bilayer graphene for a given pressure "p" perpendicular to the surface. For continuous materials the relation between...

13 February 2013 619 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View