Bernd Koeberlein

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Questions related from Bernd Koeberlein

Selection of MSCs/ECFCs with puromycin after transduction - can anyone help?

I transduce MHC and ECFC cells with a lentiviral vector for gfp expression. After selection with puromycin I get at least 50 % of the cells which don´t glow green although the selection control...

13 March 2014 750 1 View

Senescence: does anybody know how to detect senescence in stem cells without using FACS?

Stem cells undergo senescence and lose their capability to differentiate. We are looking for a method to detect at which passage this happen.

27 January 2014 8,239 3 View