Sample is fungal and coated with Au/Pd and mounted with hot glue to the sample holder.
Image drift is worse at 5kV than 10kV
The image drift is visible on the high refresh rates used to navigate and only at higher magnifications (4500x +), but does not result in artifacts at photo quality refresh rates. If the sample was actually moving it should be represented as streaking in the low refresh rates, right?
The carbon tape you are using to fix the specimen to the Sem machine is new and it has thickness and glue. If you leave your specimens for long duration under pressure but without observation so the tape and glue will be compressed and flat and will not cause you any problem.
That's odd. Usually the drift caused by charging is noticeable in all scanning speeds, although you see some distortion at lower speeds (photo quality) rather than movement. Furthermore, it is more evident in higher magnifications, since you are increasing the current density (smaller area and same current).
Did you try to use a conductive glue/paint or a carbon tape to fix your samples on the stub? I believe the hot glue is the cause of the drifting. You can also try to ground you sample with conductive paint/glue. If it is in a cover slip or other solid substrate, you can paint the edges. If it is a biological piece, you can try to paint the parts that are in contact with the hot glue and the hot glue itself. That could improve your grounding quality.
Good luck!
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