Hi,
I need an article/summary on different resolution patterns (including fall by crisis and fall by lysis).
Thanks :)
Hi fellows, I've started a project in a new lab where I have to extract RNA from mice liver, brain, and muscles. Upon dissection I immediately snap-freeze the tissue in liquid nitrogen and store...
19 February 2024 1,639 4 View
I need to extract the liver from frozen Emerald rockcod and sequence its RNA. To keep the RNA stable and prevent degradation, I would like to avoid thawing the fish for the dissection. However, I...
23 October 2023 6,131 2 View
Hi everyone, I've tried to transfer initially using a 20% methanol transfer buffer using 250mA max current for 70 mins, all I got was transfer of mid-high range proteins, I suppose all proteins...
25 July 2023 1,863 4 View
Hlw I am doing a research on Homophobia among youth and parents for which I am thinking to use a standardized questionnaire- Homosexuality Attitude Scale (HAS), but the scoring information of the...
01 June 2023 4,366 13 View
Estimada red, estoy iniciando a desarrollar un articulo, me gustaria me brinden ideas para cosiderarlas en este nuevo proyecto.
19 March 2023 7,037 1 View
Hello all, I've got a rather complex question concerning my regression. My data has the following properties. Dependent variable is count data and is overdispersed and consist of repeated...
22 February 2023 8,786 3 View
I am trying to find an article on the diversity of mould species. They are found in the divisions Zygomycota and Ascomycota. Does anyone know how many genus or estimated number of mould species...
16 February 2023 9,059 2 View
Hello, I am running a dft calculation that keeps completing in less than minute giving me this error: "No data on chk file. Error termination via Lnk1e in /apps/gaussian/g16c01/g16/l301.exe" I...
06 January 2023 2,533 2 View
Usually taqman qPCR gene expression assays using taqman fast advanced mastermix require 20ul reaction volumes. I wanted to scale down reaction volumes to 10 or 5ul to save on reagents but I was...
21 June 2021 9,273 3 View
I am at my wits end with this PCR so I am hoping someone in the world might have some ideas. I am consistently getting inconsistent results. I am following the PCR protocols adapted from "...
08 June 2021 7,972 10 View
One of my friends, every night after falling asleep, his mouth would make a creaking sound after chewing something. This teeth grinding condition would continue until he woke up, and he didn't...
08 August 2024 1,366 5 View
Hi all, I am looking for advice on what could be going wrong with my zebrafish caudal fin tissue fixation that would cause the sample to fall out of the embedding while cryosectioning. During cryo...
25 June 2024 4,602 0 View
I use a DISCO DAD323 dicing machine to cut my sample,which has 1-2 micrometers SIO2 on its top.However,the SIO2 is broken and fall apart everytime.I tried different cutting speed but didn't work....
20 June 2024 1,131 0 View
We have been waiting for over a year for pneu dart to make VHF darts available for purchase again, but they still will not provide a date. We are in bad need of darts for a deer collaring project...
29 May 2024 1,799 0 View
I have to rear the R-strain for my research
13 May 2024 3,394 0 View
i am working with gene editing in bell pepper. i have done agro transformation by floral dip method. (LBA4404 carrying plasmid, at final pellet dissolved in 5% sucrose containing 0.02% silwet ). i...
13 May 2024 7,460 6 View
What is the reason for the Moon moving away from Earth? Will it eventually leave Earth's orbit and how does a satellite orbit without falling into the Earth?
01 May 2024 8,190 0 View
I could not manage to fall into negative potential while taking GCD on Versastad 4 potentiostad. So if the CV is between -0.5 AND 1.0 V, I want to use the same potential range in the GCD. I enter...
24 April 2024 4,163 0 View
I did my lab and found out one patient got PI2 and PI4 positives due to fever. Hence, I need confirmation if anyone gets the same result as me.
24 April 2024 1,322 1 View
The Marquis de Lafayette is "returning" to America again this fall. After serving in the American and French Revolutions, he returned to America in 1824-1825 and toured some 25 states. This...
19 April 2024 4,979 0 View