Hello,

We found a protocol online to generate CM chip.

Wash it by water X 3, dry it by N2 gas. Spreaded 70 μL of 20 mM cystamine hydrochloride onto the Au film, put into a box, overnight at room temperature. Wash it by water X 3, dry it by N2 gas. Then, the 70 μL mixture solution of 15 mM EDC, 75 mM NHS, and 10 mg mL−1 CD was dropped onto the cystamine modified Au surface for 2.5 h and the Au surface was cleaned by water X 3, dry it by N2 gas, and stored at -20C.

The coupling efficiency is not good and it's difficult to find another protocol using EDC/NHS to coat dextran. Can you pls give a suggestion?

Thanks.

Shawn