Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

Can you help me with a free /no cost publication journal in oncology Nursing?

Wish to publish an article but in a cost-free journal. Need the assistance of researchers on good indexed journals free of predatory. Thank you

23 September 2020 5,372 3 View

How do you differentiate your different populations in your FASTA file in R ?

I need to identify my populations from my FASTA file in R so that R can then run all my stats. However, I do not know how to tell R how to group my data into 4 populations! This is the warning...

09 September 2020 8,875 2 View

How to generat a ASCII format data in GIS?

Dear you all: Recently i met with such a problem: i tryed to generate a ascii format buffer in gis, but i failed because it's headers are different from the research data. Failed result are shown...

11 August 2020 5,040 3 View

Do 3 light sources with same intensities, but different wavelengths(blue,green, red) provide same amount of energy for photosynthesis?

As it is not directly my field, I would like to ask for help in terms of the correlation between light intensity, wavelength, frequency and amplitude. Let's say, I have a radiophotometer which...

29 April 2020 9,141 2 View

How does one identify and extract statoliths from cubozoans?

Specifically, I am looking at the species Alatina alata. I am having trouble both finding the statoliths and also extracting them for further analysis. I have been freezing specimen before I am...

24 April 2019 7,943 2 View

Which test to use in SPSS?

So i have a study with both males and females, they will both be shown a negative image and their brain reaction recorded and a positive image with their brain reaction recorded what analysis in...

30 March 2019 4,571 6 View

Using floating dead cells in western blot analysis after drug treatment?

Hey guys, I need your help to decide if I should include floating dead cells in my western blot analysis. I am looking to check expression of certain proteins after drug treatment in adherent...

28 January 2019 4,354 10 View

Trouble with protein ladder in western blot. what could be the reason?

I am using 4-20% biorad precast mini gel and protein ladder is also from biorad. I have attached the image of membrane. Running voltage- 100 V for 1 hr at room temperature and 70 V for 2.30 hr at...

18 December 2018 4,231 5 View

How to solve a steady state homogenous problem in Matlab using Matrix approach?My results does not mimick the sytem under observation.Any suggestion?

I am solving a numerical modelling stem of autothermal reforming using MATLAB. The problem is ,Pressure and Temperature results are not stable (unsteady state problem) and escalate too much I...

06 July 2018 1,012 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View